منابع مشابه
Immunophenotyping.
This unit presents basic techniques for immunophenotyping by flow cytometry, direct using a conjugated monoclonal antibody and indirect using an unconjugated primary antibody followed by a conjugated secondary antibody. Combinations of these methods are described for two-, three-, and four-color staining. Analysis of data acquired from cells stained by these procedures is detailed. A procedure ...
متن کاملStandardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium
Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, eight-color panels for identification of major immune cell subsets in peripheral blood. These were produced as pre-configured, lyophilized, reagents...
متن کاملImmunophenotyping of acute myeloid leukemia using monoclonal antibodies and the alkaline phosphatase-antialkaline phosphatase technique.
The leukemic cells from 41 cases of acute myeloid leukemia (AML) and 17 cases of acute lymphocytic leukemia (ALL) were immunophenotyped by the alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical technique utilizing eight monoclonal antibodies (MoAb) reactive with cells of myeloid origin and seven MoAb reactive with lymphoid antigens. Ninety percent of the cases of AML react...
متن کاملImmunophenotyping of myelodysplasia.
Cell maturation anomalies in bone marrow samples have long been recognized by the patient scrutiny of morphologists, have led to detailed descriptions of possible abnormalities and later to clinically relevant classifications of myelodysplasia (MDS). That the diagnosis and fine description of MDS belong to the realm of morphologists has long been considered a prerogative although, since the ear...
متن کاملMicroglia immunophenotyping in gliomas
Microglia, once assimilated to peripheral macrophages, in gliomas has long been discussed and currently it is hypothesized to play a pro-tumor role in tumor progression. Uncertain between M1 and M2 polarization, it exchanges signals with glioma cells to create an immunosuppressive microenvironment and stimulates cell proliferation and migration. Four antibodies are currently used for microglia/...
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ژورنال
عنوان ژورنال: Der Pathologe
سال: 2008
ISSN: 0172-8113,1432-1963
DOI: 10.1007/s00292-008-1049-4